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Lipopolysaccharide‐induced production of tumour necrosis factor and interleukin‐1 is differentially regulated at the receptor level: the role of CD14‐dependent and CD14‐independent pathways
Author(s) -
NETEA M. G.,
KULLBERG B. J.,
VAN DER MEER J. W. M.
Publication year - 1998
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1998.00532.x
Subject(s) - cd14 , lipopolysaccharide , cytokine , tumor necrosis factor alpha , receptor , biology , interleukin , microbiology and biotechnology , immunology , biochemistry
Cytokine production induced via CD14‐dependent and CD14‐independent pathways was investigated in mouse peritoneal macrophages incubated with lipopolysaccharide (LPS) or lipid A. Different LPS receptors appear to be responsible for production of tumour necrosis factor‐α (TNF‐α) and interleukin‐1α (IL‐1α) and IL‐1β. TNF‐α production is essentially CD14 dependent, both in the presence or absence of plasma. In the presence of plasma, endotoxin‐induced IL‐1 production is mediated by CD14‐dependent mechanisms, while in its absence both CD14‐dependent and CD14‐independent pathways are involved. Lipid A stimulates cytokine synthesis through both CD14‐dependent and CD14‐independent mechanisms, but its action is weaker than that of LPS, indicating that the polysaccharide moiety may be necessary for proper stimulation of mouse macrophages by endotoxin.