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Expression of CD69 activation marker by endometrial granulated lymphocytes throughout the menstrual cycle and in early pregnancy
Author(s) -
VASSILIADOU N.,
BULMER J. N.
Publication year - 1998
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1998.00497.x
Subject(s) - decidua , flow cytometry , andrology , cd69 , immunohistochemistry , biology , population , staining , immunofluorescence , antigen , endometrium , in vitro , menstrual cycle , microbiology and biotechnology , pregnancy , endocrinology , immunology , fetus , immune system , antibody , t cell , medicine , hormone , biochemistry , placenta , il 2 receptor , genetics , environmental health
Flow cytometry studies of first‐trimester normal human decidua have proposed that a proportion of endometrial granulated lymphocytes (eGL), the predominant leucocyte population in early human pregnancy, co‐express CD69. The purpose of this study was to investigate CD69 expression by immunohistochemistry throughout the menstrual cycle and in first‐trimester human decidua and to determine whether CD69 expression by eGL is an in vivo characteristic or whether the antigen is acquired in vitro after tissue disaggregation and cell purification. Single immunoenzymatic and double immunofluorescence labelling of tissue sections, supplemented with double immunoenzymatic staining of purified eGL and flow cytometry, indicated that eGL do not co‐express CD69 in situ but that they acquire the molecule in vitro after cell purification and culture.