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Nitric oxide‐mediated cytotoxic effects of alveolar macrophages on transformed lung epithelial cells are independent of the β 2 integrin‐mediated intercellular adhesion
Author(s) -
Seishiro Hirano
Publication year - 1998
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1998.00393.x
Subject(s) - cytotoxicity , cytotoxic t cell , lipopolysaccharide , nitric oxide , cd18 , macrophage , chemistry , intracellular , microbiology and biotechnology , integrin , intercellular adhesion molecule 1 , alveolar macrophage , biology , immunology , in vitro , biochemistry , cell , organic chemistry
It is known that murine macrophages produce nitric oxide (NO) when stimulated with lipopolysaccharide (LPS) or interferon‐γ (IFN‐γ), and NO mediates the tumoricidal activity of activated macrophages. The present study was designed to investigate whether the intercellular adhesion is necessary for activated rat alveolar macrophages to exert the full cytotoxic effects. Rat alveolar macrophages produced NO dose dependently in response to either LPS or IFN‐γ, and caused DNA fragmentation in rat type II pneumocytes transformed with SV40 (SV40T2). Chemically produced NO also caused the DNA fragmentation and viability loss in SV40T2, and both of them were inhibited by a NO radical scavenger. The cytotoxicity of activated macrophages was reduced by N G ‐monomethyl‐l‐arginine, a competitive nitric synthase inhibitor, and neither superoxide dismutase nor catalase modulated the cytotoxicity. Although alveolar macrophages stimulated with either LPS or IFN‐γ caused DNA fragmentation of SV40T2, only LPS increased the intercellular adherence between macrophages and SV40T2. The intercellular adhesion was reduced by both anti‐CD18 and anti‐CD11a. However, those antibodies did not affect the cytotoxicity of LPS‐stimulated macrophages. These results clearly indicate that NO‐mediated cytotoxicity is caused predominantly by diffusion of NO, and the β 2 integrin‐mediated intercellular adhesion does not play an important role, if any, in activated macrophage‐mediated cytotoxic effects on SV40T2.