Premium
The role of the Fgr tyrosine kinase in the control of the adhesive properties of U937 monoblastoid cells and their derivatives
Author(s) -
FAULKNER‡ L.,
PATEL M.,
BRICKELL§ P. M.,
KATZ D. R.
Publication year - 1997
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1997.00367.x
Subject(s) - u937 cell , transfection , cell culture , biology , tyrosine kinase , microbiology and biotechnology , intracellular , cell adhesion , gene expression , cell , biochemistry , gene , signal transduction , genetics
In humans, expression of the cellular proto‐oncogene c‐ fgr is normally restricted to mature cells of the myeloid lineage, mantle zone B cells and various myeloid and B‐cell lines. Previous studies of the monoblastoid cell line, U937, showed that c‐ fgr expression increased following differentiation, but its role in monocytes and related cells has not been defined in functional terms. We therefore investigated the role of c‐ fgr in U937 cells transfected with the c‐ fgr gene such that its expression could be manipulated independent of differentiation. Induction of the transfected c‐ fgr gene by cadmium ions did not affect cell proliferation, responses to phorbol 12‐myristate 13‐acetate (PMA), dihydroxycholecalciferol (DHCC), tumour necrosis factor‐α (TNF‐α) or retinoic acid, or phagocytosis of antibody‐coated sheep red blood cells. However, there was increased surface expression of CD54 (intracellular adhesion molecule‐1; ICAM‐1) and CD102 (ICAM‐2) and decreased surface expression of CD50 (ICAM‐3) compared with cells that had been transfected with plasmid only and treated in the same way. These findings suggest that the product of the c‐ fgr gene may be important in control of relative adhesive properties of mature monocytic cells.