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Characterization of the bovine ε gene
Author(s) -
MOUSAVI M.,
RABBANI H.,
HAMMARSTRÖM L.
Publication year - 1997
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1997.00352.x
Subject(s) - gene , biology , genetics , computational biology
Immunoglobulin E is quantitatively a minor immunoglobulin class in serum, but nevertheless the major class of antibody mediating type I hypersensitivity reactions and hence, type I allergic phenomena. The bovine ε gene is one of the as yet uncharacterized mammalian immunoglobulin genes. We have therefore cloned and determined the cDNA sequence and genomic organization of the gene. It contains four constant region domain‐encoding exons (CH1 to CH4) with a high homology to sheep Cε (87%) and to a lower degree to dog (62%), horse (58%), chimpanzee, orangutan, human (55%), mouse (52%) and rat (52%) Cε genes. Southern blot analysis of bovine genomic DNA, revealed the existence of a single Cε gene with a presence of allelic restriction fragment length polymorphism (RFLP).