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A nitric oxide‐releasing reagent, S‐nitroso‐N‐acetylpenicillamine, enhances the expression of superoxide dismutases mRNA in the murine macrophage cell line RAW264‐7
Author(s) -
SANO H.,
HIRAI M.,
SAITO H.,
NAKASHIMA I.,
ISOBE K.I.
Publication year - 1997
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1997.00322.x
Subject(s) - superoxide dismutase , snap , nitric oxide , microbiology and biotechnology , chemistry , cell culture , nitric oxide synthase , penicillamine , dismutase , macrophage , sod1 , biochemistry , biology , oxidative stress , computer graphics (images) , genetics , organic chemistry , computer science , in vitro
Murine interferon‐& ggr; (IFN‐& ggr; ) stimulates the murine macrophage tumour cell line RAW264‐7 to produce nitric oxide (NO). IFN‐& ggr; induces expression of inducible NO synthase (iNOS), manganese superoxide dismutase (Mn‐SOD) and copper zinc SOD (CuZn‐SOD) in these cells. To investigate the mechanism of induction of SOD expression, we added S‐nitroso‐N‐acetyl penicillamine (SNAP) to RAW264‐7 cells. SNAP enhanced the expression of Mn‐SOD and CuZn‐SOD. These results suggest that when producing NO, RAW264‐7 cells express SOD that might protect them from NO toxicity.