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Differential expression of CD8 epitopes amongst porcine CD8‐positive functional lymphocyte subsets
Author(s) -
YANG H.,
PARKHOUSE R. M. E.
Publication year - 1997
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1997.00308.x
Subject(s) - cd8 , epitope , biology , immunology , lymphocyte subsets , lymphocyte , cytotoxic t cell , immune system , antigen , genetics , in vitro
The swine is a useful model for immunobiological studies as it has a highly heterogeneous lymphocyte pool, containing several subsets not easily accessible in humans and rodents. In particular, the CD8‐positive (CD8 + ) cells contain a variety of lymphocyte subsets, such as αβ‐T cells, γδ‐T cells, CD4 CD8 double‐positive (DP) cells and natural killer (NK) cells. In order to define these subsets further, we have selected four monoclonal antibodies (mAb) with differential reactivity on CD8 + cells. Thus, mAb CD8.1 (PPT20) bound to CD8 hi and CD8 lo subpopulations in a similar way to the conventional anti‐CD8. The mAb CD8.2 (PPT21), though binding to all of the CD8 + cells, reacted preferably with CD8 hi . Two other mAb, CD8.3 (PPT22) and CD8.4 (PPT23), were specific for CD8 hi αβ‐T‐cell subpopulation. These results, complemented by immunoprecipitation, co‐modulation and enzyme‐linked immunosorbent assay experiments, suggest that CD8.1 and CD8.2 react putatively with the CD8α‐chain and CD8.3 and CD8.4 with the CD8β‐chain. Tissue distribution studies revealed that CD8 + thymocytes and peripheral CD8 hi αβ‐T cells expressed both putative CD8α‐ and β‐chains while peripheral CD4 + CD8 + αβ‐T cells, CD8 lo γδ‐T cells and NK cells expressed only putative CD8α‐chain. Functional studies indicated that the CD8 hi αβ‐T and CD8 lo γδ‐T cells were effector cells in the CD3‐redirected cytotoxicity.