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HIV‐1‐specific cell‐mediated immune responses induced by DNA vaccination were enhanced by mannan‐coated liposomes and inhibited by anti‐interferon‐γ antibody
Author(s) -
TODA S.,
ISHII N.,
OKADA E.,
KUSAKABE K.I.,
ARAI H.,
HAMAJIMA K.,
GORAI I.,
NISHIOKA K.,
OKUDA K.
Publication year - 1997
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1997.00307.x
Subject(s) - mannan , dna vaccination , immune system , liposome , adjuvant , antibody , immunopotentiator , immunology , biology , virology , vaccination , interferon , immunization , biochemistry , polysaccharide
The adjuvant effect of mannan‐coated liposomes on human immunodeficiency virus type‐1 (HIV‐1) DNA vaccine and the mechanism of this enhancement were studied. Coating of cationic liposomes with mannan significantly enhanced the ability of this vaccine to induce an HIV‐specific delayed‐type hypersensitivity (DTH) response. HIV‐specific cytotoxic T‐cell (CTL) activity elicited by DNA vaccination was also significantly enhanced with the mannan‐liposome cocktail. This mannan‐liposome‐mediated activity was greatly inhibited by in vivo injection of anti‐interferon (IFN)‐γ antibody, which suggests that IFN‐γ plays an important role in this HIV‐specific immune response. The results of both isotype‐specific antibody and cytokine analysis revealed that mannan‐liposome‐mediated DNA vaccination enhances Th1‐mediated immunity.