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Subunit composition of the pre‐T‐cell receptor complex analysed by monoclonal antibody against the pre‐T‐cell receptor α chain
Author(s) -
KOSUGI A.,
NODA S.,
SAITOH S.I.,
NARUMIYA S.,
OGATA M.,
HASHIMOTO Y.,
TAKASE K.,
SAITO T.,
HAMAOKA T.
Publication year - 1997
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1997.00294.x
Subject(s) - monoclonal antibody , interleukin 5 receptor alpha subunit , interleukin 10 receptor, alpha subunit , receptor , microbiology and biotechnology , g alpha subunit , alpha (finance) , antibody , protein subunit , chemistry , alpha chain , biology , biochemistry , immunology , medicine , gene , construct validity , nursing , patient satisfaction
The pre‐T‐cell receptor (TCR) complex, which consists of a heterodimer of the TCR β ‐chain and the pre‐TCR α ‐chain, is known to regulate early thymocyte development. The pre‐TCR complex contains CD3 subunits as a signal‐transducing molecule, but the exact subunit composition of the fully assembled pre‐TCR complex remains to be elucidated. In particular, the association of the CD3 ζ ‐chain with the pre‐TCR is controversial. In the present study, we have generated a monoclonal antibody against the cytoplasmic portion of the pre‐TCR α ‐chain, and analysed a subunit composition of the pre‐TCR complex. We demonstrated that the CD3 ζ ‐chain is physically associated with the pre‐TCR in immature T cells. Thus, the result strongly supports the previous findings that CD3 ζ contributes to signalling mediated through the pre‐TCR complex.