Premium
Human & gamma; δ T‐cell recognition of Yersinia enterocolitica
Author(s) -
YOUNG J. L.,
GOODALL J. C.,
BEACOCKSHARP H.,
HILL GASTON J. S.
Publication year - 1997
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1997.00289.x
Subject(s) - yersinia enterocolitica , t cell , peripheral blood mononuclear cell , cytotoxic t cell , biology , cell culture , immunology , yersinia , cd8 , gamma delta t cell , antigen , t cell receptor , microbiology and biotechnology , virology , immune system , bacteria , biochemistry , genetics , in vitro
We have studied the human & gamma; & delta; T‐cell response to Yersinia enterocolitica , a facultative intracellular bacterium which causes gastroenteritis and, particularly in human leucocyte antigen (HLA)‐B27 + individuals, reactive arthritis (ReA). A marked proliferation of that cytotoxic & gamma; & delta; T cells is seen when Yersinia ‐infected lymphoblastoid cell lines or fixed intact Yersinia are added to cultures of mononuclear cells derived from the synovial fluid of ReA patients or from the peripheral blood of healthy donors. In contrast, heat‐inactivated Yersinia fail to stimulate the & gamma; & delta; T‐cell response. The & gamma; & delta; T‐cell lines generated killed both autologous and allogeneic infected cell lines. Interestingly, a T‐cell line generated from synovial fluid mononuclear cells (SFMC) killed infected autologous cell lines and a cell line matched for HLA‐B27 less well than infected allogeneic target cells. & gamma; & delta; T‐cell clones isolated from this line were found to express V & gamma;9V & delta;2 T‐cell receptor (TCR) and also killed infected mismatched cells more efficiently than autologous targets. Moreover, from experiments using major histocompatability complex (MHC)‐deficient cell lines, it was apparent that target cell recognition was MHC independent. Our results suggest that & gamma; & delta; T cells can be involved in immunity to Yersinia enterocolitica and should be taken into account when considering immunopathological mechanisms leading to reactive arthritis.