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Treatment with anti‐LFA‐1 α monoclonal antibody selectively interferes with the maturation of CD4 − 8 + thymocytes
Author(s) -
REVILLA C.,
GONZÁLEZ A. L.,
CONDE C.,
LÓPEZHOYOS M.,
MERINO J.
Publication year - 1997
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1997.00183.x
Subject(s) - thymocyte , cd8 , t cell receptor , biology , t lymphocyte , t cell , cd3 , microbiology and biotechnology , monoclonal antibody , major histocompatibility complex , stromal cell , antigen , antibody , immunology , immune system , cancer research
Maturation of T lymphocytes in the thymus is driven by signals provided by soluble factors and by the direct interaction between thymocytes and stromal cells. Although the interaction between T‐cell receptor (TCR) and major histocompatibility complex (MHC) molecules on stromal cells is crucial for T‐cell development, other accessory molecules seem to play a role in this process. In order to better understand the role of lymphocyte function‐associated antigen‐1 (LFA‐1) and intercellular adhesion molecule‐1 (ICAM‐1) molecules in thymocyte maturation, mice were treated from birth with saturating doses of non‐cytolytic‐specific monoclonal antibodies. The effect of this treatment on thymocyte subpopulations and the expression of CD3 and TCR‐ αβ by these cells was investigated by flow cytometry. Our data demonstrated that the effective saturation of LFA‐1 α chain in the thymus, but not ICAM‐1 or LFA‐1 β chain, selectively interfered with the maturation of CD8 + T cells, as manifested by a marked reduction in the frequency of CD4 − 8 + thymocytes expressing high levels of CD3 and TCR‐ αβ . This selective reduction was also observed in peripheral blood mononuclear cells and spleen cells. The analysis of the frequencies of various V β TCR showed that CD4 − 8 + thymocytes were globally affected by the treatment. These results underline the importance of the interaction between LFA‐1 and its ligands in the maturation of CD8 + T cells and document the existence of different molecular requirements for the differentiation of CD4 + and CD8 + T cells.