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Antigen‐presenting cell‐derived signals determine expression levels of CD70 on‘qc primed T cells
Author(s) -
LENS S. M. A.,
BAARS P. A.,
HOOIBRINK B.,
VAN OERS M. H. J.,
VAN LIER R. A. W.
Publication year - 1997
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1997.00134.x
Subject(s) - priming (agriculture) , biology , cd28 , antigen , t cell , immune system , interleukin 21 , antigen presenting cell , microbiology and biotechnology , immunology , botany , germination
Interaction between CD27 and its ligand CD70 provides a second signal for T‐cell proliferation and tumour necrosis factor‐ α (TNF‐ α ) production. Whereas CD27 is broadly expressed during T‐cell development, expression of CD70 in vivo is restricted. To determine when CD27–CD70 interactions can occur in immune reactions, we here analysed the regulation of CD70 expression on activated T cells. Mitogenic stimulation of purified T cells with either immobilized CD3 monoclonal antibody (mAb) or a combination of CD2 mAb induces only low levels of CD70 membrane expression. Markedly, expression of the CD27‐ligand is strongly enhanced by antigen‐presenting cells (APC) and APC‐associated signals such as interleukin‐1 α (IL‐1 α ), IL‐12, TNF‐ α and CD28‐ligation. In contrast, T‐cell derived cytokines, such as IL‐4, counteract CD70 up‐regulation on activated T cells. Analysis of the small subset of circulating CD70 + T cells revealed that these cells have a primed phenotype as they express CD45RO and HLA‐DR antigens and are in high frequency able to secrete interferon‐ γ (IFN‐ γ ). We conclude that T–T interactions involving CD27 and CD70 are likely to occur relatively early in immune reactions, after productive T‐cell priming by APC and that expression of CD70 on circulating T cells is a reflection of recent priming by antigen.