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DIFFERENTIAL EXPRESSION OF FUNCTION‐RELATED ANTIGENS ON NEWBORN AND ADULT MONOCYTE SUBPOPULATIONS
Author(s) -
MURPHY F. J.,
REEN D. J.
Publication year - 1996
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1996.d01-788.x
Subject(s) - cd14 , monocyte , cord blood , antigen , cd16 , biology , immunology , umbilical cord , immune system , cd8 , cd3
Umbilical cord blood and adult peripheral blood monocytes were separated into two subpopulations, based on the intensity of CD14 expression, and the coexpression of various antigens associated with monocyte function was examined. The majority of cord and adult monocytes expressed CD14 at a high density (CD14 bright ) while approximately 15% of monocytes expressed this antigen at a lower level (CD14 dim ). Three times as many CD14 dim monocytes expressed CD16 (FcγRIII) as did CD14 bright monocytes in both cord and adult preparations, while its level of expression (mean fluorescence intensity) was significantly reduced on cord CD14 dim monocytes relative to adult CD14 dim monocytes. The percentage of cord blood CD14 dim monocytes expressing human leucocyte antigen‐DR (major histocompatibility complex class II antigen) was significantly reduced relative to adult CD14 dim cells; however, the level of expression of this antigen was greater on both cord and adult CD14 dim monocytes compared with CD14 bright cells. Cord CD14 bright cells expressed CD36 (OKM5) to a greater extent than did their adult counterparts. The level of expression of CD62L was reduced on cord CD14 dim cells compared with adult CD14 dim cells. CD11b (CR3) was expressed both on a higher percentage of cells and also with a greater intensity on both cord and adult CD14 bright monocytes compared with their CD14 dim counterpart. These results show that significant differences exist between cord and adult monocyte subpopulations with regard to expression of various antigens associated with specific effector function.