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Completely allogeneic spleen cells induced cytolytic neonatal tolerance to alloantigens, but failed to establish allo‐helper interactions with host T cells
Author(s) -
RAMOS A.,
GONZÁLEZ M.,
LÓPEZHOYOS M.,
CARRIÓ R.,
MERINO J.
Publication year - 1996
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1996.d01-763.x
Subject(s) - spleen , immunology , biology , antigen , antibody , major histocompatibility complex , cytolysis , microbiology and biotechnology , in vitro , cytotoxic t cell , genetics
The injection of spleen cells from F 1 mice into newborns from a parental strain results in the establishment of cytolytic tolerance to donor alloantigens and the development of a lupus‐like disease. This syndrome is the consequence of the recognition by alloreactive host CD4 + T cells of discordant major histocompatibility complex (MHC) class II antigens on semi‐allogeneic donor B cells. We have analysed whether completely allogeneic spleen cells are as able as semi‐allogeneic spleen cells to induce cytolytic tolerance to donor alloantigens and to co‐operate with alloreactive T cells for autoantibody production. BALB/c mice were injected at birth with Thy‐1‐depleted spleen cells from (C57BL/6 × BALB/c)F 1 or C57BL/6 mice, either alone or in combination. Cytolytic tolerance was always induced, as manifested by persistence of chimerism and acceptance of skin allografts. However, only F 1 semi‐allogeneic B cells were activated by alloreactive host T cells to produce anti‐DNA IgG antibody. The deficient co‐operation between BALB/c CD4 + T cells and completely allogeneic C57BL/6 B cells was confirmed after neonatal injection of (C57BL/6 × BALB/c)F 1 (Igh a ) spleen cells together with C57BL/6 (Igh b ) spleen cells. These mice developed anti‐DNA antibodies bearing only the Igh a allotype. Similar results were observed in experiments of allogeneic interaction in vitro , in which BALB/c CD4 + T cells were cocultured with either (C57BL/6 × BALB/c)F 1 or C57BL/6 B cells. The present results demonstrate that completely allogeneic spleen cells efficiently induced cytolytic unresponsiveness to donor alloantigens, but B cells contained in this spleen cell population were unable to establish allo‐helper interactions with alloreactive CD4 + T cells, suggesting that cytolytic and helper T‐cell interactions involved in alloreactivity may be different.