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COSTIMULATION REGULATES THE KINETICS OF interleukin‐2 RESPONSE TO BACTERIAL SUPERANTIGENS
Author(s) -
MURAILLE E.,
DEVOS S.,
THIELEMANS K.,
URBAIN J.,
MOSER M.,
LEO O.
Publication year - 1996
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1996.d01-734.x
Subject(s) - cd80 , superantigen , cd86 , t cell , biology , antigen , antigen presenting cell , immune system , microbiology and biotechnology , monoclonal antibody , immunology , cd40 , in vitro , antibody , cytotoxic t cell , biochemistry
The aim of this study was to investigate the mechanisms by which B7‐related costimulatory molecules (CD80, CD86) increase T‐cell responsiveness to extracellular ligands. As a model study, the in vitro response of purified splenic CD4 + T cells to a bacterial superantigen, SEB, was characterized. Previous analysis of this experimental model led us to conclude that expression of B7‐related molecules is strictly required in order to activate CD4 + T cells in the presence of bacterial superantigens. In the present report, we demonstrate that antigen‐presenting cell‐derived costimulatory signals regulate the kinetics of interleukin‐2 (IL‐2) production by SEB‐activated splenic CD4 + T cells. Indeed, experiments performed with purified subpopulations of antigen‐presenting cells and using B7‐transfected cell lines indicated that increased levels of CD80 and/or CD86 cell surface expression is associated with a faster kinetics of IL‐2 production in response to SEB. Accordingly, blocking of CD80 or CD86‐derived signals by specific monoclonal antibodies led to a slower kinetics of IL‐2 production in response to SEB. Thus these data demonstrate that similar strength of signal through the T‐cell receptor can lead to immune responses displaying distinct kinetics depending on the level of costimulatory ligands on APC.