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Synthetic peptides non‐covalently bound to bacterial hsp 70 elicit peptide‐specific T‐cell responses in vivo
Author(s) -
ROMÁN E.,
MORENO C.
Publication year - 1996
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1996.d01-697.x
Subject(s) - immunogenicity , peptide , adjuvant , heat shock protein , in vivo , chaperone (clinical) , immunization , immune system , biology , t cell , microbiology and biotechnology , chemistry , biochemistry , virology , immunology , medicine , pathology , gene
We have examined the immunogenicity of complexes formed by non‐covalent association of a synthetic peptide corresponding to influenza A virus nucleoprotein, residues 206–229 (pNP) and Mycobacterium tuberculosis heat‐shock protein 70 (hsp 70). One or two injections of these complexes given to BALB/c mice without any additional adjuvant, were capable of eliciting very strong peptide‐specific proliferative T‐cell responses in the spleen. These responses were dependent on the stability of the complex since immunogenicity was lost when dissociated with ATP prior to immunization. T‐cell responses to hsp 70 were easily generated by immunization with the purified chaperone alone, either after primary or secondary immunization. Injection of pNP–hsp 70 complexes, however, although generating good primary responses, resulted in very much decreased proliferative responses to the hsp 70.