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Chemotaxis of human B lymphocytes to anti‐IgD
Author(s) -
KOMAIKOMA M.,
WILKINSON P. C.
Publication year - 1996
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1996.d01-686.x
Subject(s) - immunoglobulin d , chemotaxis , receptor , biology , immunology , antigen , population , endocrinology , medicine , antibody , b cell , biochemistry , environmental health
The resting population of small surface IgM + and surface IgD + B cells from the human tonsil can be preactivated by overnight culture in interleukin‐4 (IL‐4) to show locomotor responses to anti‐IgM and anti‐IgD at between 10 ng and 1 μg/ml. Because this locomotion is activated through the antigen receptor and may simulate a response to antigen, we set out to establish whether this was a chemotactic response using a checkerboard filter assay with a range of concentrations and concentration gradients of anti‐IgD. At high concentrations (100 ng/ml to 1 μg/ml), a chemokinetic response, but no chemotaxis, to anti‐IgD was seen. However, in concentration gradients set up at lower concentrations (0–50 ng/ml) a chemotactic response was demonstrable. During the period of culture in anti‐IgD at 1 μg/ml, a progressive loss of surface IgD from the cells was seen, but there was no loss at 10 ng/ml. This receptor loss from the cell surface may account for the lack of chemotactic effect of the anti‐IgD at higher concentrations.