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Modulation of WC1, a lineage‐specific cell surface molecule of γ/δ T cells, augments cellular proliferation
Author(s) -
HANBYFLARIDA M. D.,
TRASK O. J.,
YANG T. J.,
BALDWIN C. L.
Publication year - 1996
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1996.d01-649.x
Subject(s) - monoclonal antibody , microbiology and biotechnology , antigen , scavenger receptor , t cell , receptor , antibody , biology , chemistry , immunology , biochemistry , cholesterol , immune system , lipoprotein
WC1, also known as T19, is the only unique γ/δ T‐cell differentiation antigen described to date other than the γ/δ T‐cell receptor. We present evidence that modulation of WC1 results in augmented proliferation of γ/δ T cells. Immobilized IL‐A29, a monoclonal antibody (mAb) specific for WC1, augmented proliferation of γ/δ T cells in the autologous mixed leucocyte reaction (AMLR) as well as proliferation induced by either anti‐CD3 or anti‐CD5 mAb. In contrast, anti‐CD5 mAb did not increase proliferation in the AMLR even though both CD5 and WC1 are members of the scavenger receptor cysteine‐rich family of proteins and are expressed by bovine peripheral blood γ/δ T cells. IL‐A29 did not induce proliferation when assessed alone or in the presence of either phorbol myristate acetate (PMA) or interleukin‐2. IL‐A29 also did not induce detectable calcium mobilization when evaluated in the presence of monocytes, PMA, or following cross‐linking of IL‐A29 with anti‐immunoglobulin antibody. We conclude that WC1 is a γ/δ T‐cell lineage‐specific cell‐surface differentiation antigen which is involved in activation of γ/δ T cells using an as yet unidentified pathway.