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Expression of functional molecules by human CD3 − decidual granular leucocyte clones
Author(s) -
GUDELJ L.,
DENIZ G.,
RUKAVINA D.,
JOHNSON P. M.,
CHRISTMAS S. E.
Publication year - 1996
Publication title -
immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.297
H-Index - 133
eISSN - 1365-2567
pISSN - 0019-2805
DOI - 10.1046/j.1365-2567.1996.508583.x
Subject(s) - cd3 , biology , microbiology and biotechnology , cd16 , receptor , monoclonal antibody , antigen , antibody , immunology , cd8 , biochemistry
Cell surface and cytoplasmic antigen expression by 35 CD3 − decidual granular leucocyte (DGL) clones, derived from human endometrial tissue in the first trimester of pregnancy, has been compared with both that of fresh CD3 − decidual leucocytes and that of CD3 − peripheral blood natural killer (PBNK) cell clones ( n = 12). The majority of DGL clones retained the antigenic phenotype of fresh cells, although CD103 (HML‐1) was expressed on 50% of DGL clones but only 17% of fresh DGL. Both cytoplasmic CD3ζ and CD3ε chains were detected in > 90% of DGL clones in the absence of cell surface CD3. Cytoplasmic CD3ζ was present in almost all fresh CD3 − DGL, whereas CD3ε was not. Most DGL clones did not express surface Fcγ receptors I‐III (CD64, ‐32 and ‐16, respectively) and complement receptors (CR) types 1 and 2 (CD35 and 21, respectively), but 43% expressed CR3 (CD11b/18); in contrast, all PBNK clones were CR3 + . The NK cell‐associated molecules Kp43 (CD94) and the p58 molecule recognized by the HP3E4 monoclonal antibody were both present on a higher proportion of CD3 − PBNK (91% and 50%, respectively) than DGL clones (31% and 14%, respectively), despite expression of CD94 by > 90% of fresh CD56 + decidual leucocytes. Five of 35 CD3 − DGL clones expressed cytoplasmic CD3ζ in the absence of expression of CD2, CD16 or the p58 molecule recognized by HP3E4. These variations between CD3 − DGL and PBNK cell clones in expression of functional molecules may be related to previously reported differences in major histocompatibility complex‐non‐restricted cytotoxic activities between these two cell types.