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Expression of cyclin A and topoisomerase IIα of oligodendrogliomas is correlated with tumour grade, MIB‐1 labelling index and survival
Author(s) -
Park SH,
Suh YL
Publication year - 2003
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1046/j.1365-2559.2003.01597.x
Subject(s) - topoisomerase , cyclin d1 , oligodendroglioma , biology , immunohistochemistry , pathology , cancer research , glioma , medicine , cancer , cell cycle , immunology , astrocytoma , enzyme , biochemistry , genetics
Aims: This study was designed to investigate immunoexpression of cyclin A and D1, and topoisomerase IIα in oligodendrogliomas and to evaluate the correlation with MIB‐1 (Ki67), tumour grade, and survival of the patients. Methods and results: Forty cases of oligodendrogliomas (20 high‐ and 20 low‐grade) were studied immunohistochemically with the above‐mentioned monoclonal antibodies. Results: Normal brain tissues included in tumour sections did not express any of cyclin A, MIB‐1 and topoisomerase IIα except cyclin D1, which was shown in perineuronal and interfascicular normal oligodendroglial cells. In low‐grade and high‐grade oligodendrogliomas, the mean cyclin A labelling index (LI) was 1.18 ± 0.98% versus 4.65 ± 1.99%, respectively; the mean topoisomerase IIα LI was 1.32 ± 1.04% versus 6.63 ± 4.31%, respectively; and the mean MIB‐1 LI was 1.69 ± 1.55% versus 9.46 ± 4.66%, respectively. Interestingly, cyclin D1 was not expressed in any oligodendrogliomas. Both cyclin A and topoisomerase IIα LI showed a significant positive correlation with MIB‐1 LI and histological grade of oligodendrogliomas ( P < 0.01) and an inverse correlation with overall survival ( P < 0.01). Univariate analysis showed that cyclin A and topoisomerase IIα LIs with a cut‐off point at 3% were a significant prognostic factor ( P : cyclin A = 0.0040, topoisomerase IIα = 0.0033). Conclusion: Cyclin A and topoisomerase IIα expression are closely correlated with anaplastic oligodendrogliomas and worse clinical outcomes. Cyclin D1 seems not to be involved in the tumorigenesis of oligodendrogliomas.