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A method to fix lipids for staining fat embolism in paraffin sections
Author(s) -
Tracy R E,
Walia P
Publication year - 2002
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1046/j.1365-2559.2002.01414.x
Subject(s) - staining , adipose tissue , atheroma , oil red o , frozen section procedure , chemistry , fat embolism , linoleic acid , fatty acid , pathology , biochemistry , medicine , adipogenesis
A method to fix lipids for staining fat embolism in paraffin sectionsAims : To develop a method to preserve lipids in formalin‐fixed tissues for staining in paraffin sections, and to illustrate its use in lung and brain of a fat embolism case, and in examples of fatty liver and atheroma. Methods and results : A saturated solution of linoleic acid in 70% ethylene glycol was prepared and tissues were exposed to this for 3 days at 56°C. These tissues were treated with 2% chromic acid at 4°C for 24 h followed by 24 h in 5% sodium bicarbonate, with appropriate rinsing between solutions. Paraffin sections of these tissues were stained with a lipid‐soluble dye such as Oil Red O. Examples of fat embolism, fatty liver, and atheroma were shown photographically as illustrations of expected results. Conclusions : The demonstration of fat embolism with good quality tissue detail is made practical by the method, which is convenient and inexpensive. The method appears to be generally applicable to tissue lipids of various sorts, as exemplified by adipose tissue, fatty liver, and atheroma.