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Evidence for local immunosuppression and demonstration of c‐myc amplification in pyothorax‐associated lymphoma
Author(s) -
Yamato H,
Ohshima K,
Suzumiya J,
Kikuchi M
Publication year - 2001
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1046/j.1365-2559.2001.01197.x
Subject(s) - lymphoma , biology , immunosuppression , comparative genomic hybridization , diffuse large b cell lymphoma , cd8 , pathology , cancer research , immune system , immunology , medicine , gene , genetics , chromosome
Evidence for local immunosuppression and demonstration of c‐myc amplification in pyothorax‐associated lymphomaAims : Pyothorax‐associated lymphoma (PAL) develops in the pleural cavity of patients with a long history of pyothorax. Epstein–Barr virus (EBV) is also involved in PAL, similar to lymphomas in immunodeficient patients. Here we examined T‐lymphocyte subsets as well as c‐myc and REL gene amplification in PAL tissues. Methods and results : We determined the number and distribution of CD4+ and CD8+ T‐lymphocytes, to evaluate T‐cells in the host immune reaction in seven cases of PAL. As controls, we also studied 10 cases of extranodal diffuse large B‐cell lymphoma (DLBL) and 10 cases of nodal DLBL. Chromosomal imbalances in PAL were determined by using comparative genomic hybridization (CGH) analysis. The mean numbers of CD4+ and CD8+ and their ratio were significantly lower in PAL than in nodal DLBL. CGH analysis of PAL showed amplification of the 8q24 chromosomal region. In addition, c‐myc amplification was found in four cases of PAL by Southern blot analysis. Conclusions : Our results suggest that the development of PAL may involve a local immunosuppressive environment and that amplification of c‐myc might promote tumour progression, as has been described in the development of Burkitt’s lymphoma.