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Tenascin labelling in colorectal biopsies: a useful marker in the diagnosis of collagenous colitis
Author(s) -
I Anagnostopoulos,
Detlef Schuppan,
Ernst–Otto Riecken,
Ulrich Groß,
Harald Stein
Publication year - 1999
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1046/j.1365-2559.1999.00620.x
Subject(s) - collagenous colitis , medicine , pathology , tenascin , colitis , tenascin c , immunohistochemistry , extracellular matrix , staining , biopsy , gastroenterology , biology , fibronectin , microbiology and biotechnology
Aims This study was undertaken to clarify whether immunohistological detection of tenascin (TN), an extracellular matrix glycoprotein that is expressed during stromal remodelling, may allow a more precise diagnosis of collagenous colitis. Methods and results We studied multiple colorectal biopsies specimens from 15 patients with clinically suspected collagenous colitis for TN expression by using a monoclonal antibody. Biopsies from further 15 patients without symptoms and signs of collagenous colitis served as controls. In seven of the 15 cases with clinically suspected collagenous colitis a prominent and selective subepithelial tenascin expression was identified. The TN expression pattern closely correlated with the conventional detection of a subepithelial collagen band diagnostic of collagenous colitis. The immunohistological labelling for TN allowed a quicker and more precise measurement of the thickness of the diagnostic collagen deposits than conventional staining. By this approach one further case could be reclassified as collagenous colitis. Conclusions Our data show that immunohistological detection of TN allows a more correct and easy diagnosis of collagenous colitis.