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Distinct clonal origin of low‐grade MALT‐type and high‐grade lesions of a multifocal gastric lymphoma
Author(s) -
András Matolcsy,
Mariangy,
N Kisfaludy,
G Kelényi
Publication year - 1999
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1046/j.1365-2559.1999.00568.x
Subject(s) - malt lymphoma , lymphoma , immunoglobulin heavy chain , polymerase chain reaction , biology , gene , pathology , b cell , antibody , gene rearrangement , gastric lymphoma , mucosa associated lymphoid tissue , stomach , microbiology and biotechnology , immunology , genetics , medicine , biochemistry
Aims Low‐grade mucosa‐associated lymphoid tissue (MALT) lymphoma and high‐grade B‐cell non‐Hodgkin's lymphoma (NHL) of the stomach may occur simultaneously. To determine the clonal relationship between these tumours, we compared the immunoglobulin heavy chain gene ( IgH ) rearrangements of low and high‐grade components of a multifocal gastric NHL. Methods and results The complementary determining region 3 (CDR3) of the IgH gene rearrangements were polymerase chain reaction (PCR) amplified, cloned and sequenced. The analysis of the CDR3 sequences rearranged by tumour cells of low‐grade MALT and the high‐grade NHL revealed different nucleic acid sequences. Conclusion These findings suggest that low‐grade MALT and high‐grade B‐cell components of multifocal gastric NHL may represent unrelated clones.