Premium
Relationship between interleukin‐6 and proliferation and differentiation in cholangiocarcinoma
Author(s) -
Sugawara H,
Yasoshima M,
Katayanagi K,
Kono N,
Watanabe Y,
Harada K,
Nakanuma Y
Publication year - 1998
Publication title -
histopathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.626
H-Index - 124
eISSN - 1365-2559
pISSN - 0309-0167
DOI - 10.1046/j.1365-2559.1998.00445.x
Subject(s) - autocrine signalling , immunohistochemistry , adenocarcinoma , carcinoma , biology , pathology , cytoplasm , in situ hybridization , metastasis , cancer research , cell culture , cancer , gene expression , medicine , immunology , gene , biochemistry , genetics
Aims Interleukin‐6 (IL‐6) has been implicated as a mediator of growth control in several human neoplasms. The significance of IL‐6 expression in human cholangiocarcinoma was examined in this study. Methods and results IL‐6 expression was examined in 43 surgically resected cholangiocarcinomas and a cholangiocarcinoma cell line CCKS1, derived from abdominal metastasis of moderately differentiated adenocarcinoma, by immunohistochemical and in‐situ hybridization techniques. In non‐neoplastic bile ducts, IL‐6 was constitutively but weakly expressed. In surgical cases of cholangiocarcinoma, IL‐6 was frequently and strongly expressed in the cytoplasm of well‐differentiated cholangiocarcinoma, while its expression was decreased, and less intense or absent in moderately and poorly differentiated areas, respectively. IL‐6 mRNA was detected in the cytoplasm of carcinoma cells of two cases of cholangiocarcinomas positive for IL‐6. IL‐6 was detected in hepatic bile from two cholangiocarcinoma cases studied. The proliferation antigen Ki67 was found to be more frequently expressed in IL‐6 negative carcinoma cells than in IL‐6 positive carcinoma cells ( P < 0.01). In cultured carcinoma cells line CCKS1, IL‐6, IL‐6 mRNA and IL‐6 receptor alpha chain were detected in the cytoplasm of carcinoma cells, suggesting an autocrine effect of IL‐6 on carcinoma cells. Conclusion IL‐6 expression is inversely related to cell proliferation and positively related to differentiation in cholangiocarcinoma.