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Thrombopoietin‐induced CXC chemokines, NAP‐2 and PF4, suppress polyploidization and proplatelet formation during megakaryocyte maturation
Author(s) -
Oda Masaaki,
Kurasawa Yasuhiro,
Todokoro Kazuo,
Nagata Yuka
Publication year - 2003
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.2003.00610.x
Subject(s) - megakaryocyte , thrombopoietin , biology , thrombopoiesis , microbiology and biotechnology , bone marrow , chemokine , nap , cxc chemokine receptors , progenitor cell , immunology , haematopoiesis , inflammation , stem cell , chemokine receptor , neuroscience
Background: We previously reported that the expressions of two CXC chemokines, neutrophil activating peptide‐2 (NAP‐2) and platelet factor‐4 (PF‐4), were induced by megakaryocyte‐specific cytokine thrombopoietin (TPO) in mouse bone marrow megakaryocytes. The roles of these chemokines on megakaryocyte maturation/differentiation processes, including polyploidization and proplatelet formation (PPF) remain unresolved. Results: NAP‐2 and PF‐4 suppressed the PPF of mature megakaryocytes freshly prepared from mouse bone marrow as well as that of the megakaryocyte progenitors, c‐Kit + CD41 + cells, isolated from mouse bone marrow and cultured with TPO. NAP‐2 and PF‐4 inhibited polyploidization of c‐Kit + CD41 + cells in the presence of TPO, and also inhibited the proliferation of c‐Kit + CD41 + cells. Conclusions: NAP‐2 and PF‐4 produced by TPO stimulation in megakaryocytes suppress megakaryocyte maturation and proliferation as a feedback control.