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lal‐1 : a differentially expressed novel gene during proliferation in liver regeneration and in hepatoma cells
Author(s) -
Della Fazia Maria Agnese,
Piobbico Danilo,
Bartoli Daniela,
Castelli Marilena,
Brancorsini Stefano,
Viola Magni Mariapia,
Servillo Giuseppe
Publication year - 2002
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.2002.00593.x
Subject(s) - biology , liver regeneration , suppression subtractive hybridization , regeneration (biology) , gene , cell growth , microbiology and biotechnology , gene expression , cell cycle , hepatocyte , cdna library , genetics , in vitro
Background: During liver regeneration, 95% of the resting hepatocytes enter in G1/S phase of the cell cycle. A number of hormones, growth factors and cytokines were identified that activate signal transduction pathways playing a primary role in hepatocyte proliferation. A wide and representative cDNA library containing 1.5 × 10 6 independent clones was constructed from regenerating liver in order to identify and characterize gene the products which play a crucial role in the first hours of the proliferative process of liver regeneration. Results: A novel gene expressed in liver regeneration was cloned by subtractive hybridization. The putative protein displays in the N′‐terminal a annexin‐like domain and an aminopeptidase domain. We named the novel gene L iver A nnexin L ike‐ 1 ( lal‐1 ). The lal‐1 gene is modulated during liver regeneration, in hepatoma cells following physiological stimulation and after cAMP induction. Conclusion: The results indicate that lal‐1 is involved in liver regeneration and that its expression is finely regulated during proliferative process. The isolation of lal‐1 paves the way for a further characterization helping to assess lal‐1 involvement in cell function and proliferation.