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Cut1/separase C‐terminus affects spindle pole body positioning in interphase of fission yeast: pointed nuclear formation
Author(s) -
Nakamura Takahiro,
Nagao Koji,
Nakaseko Yukinobu,
Yanagida Mitsuhiro
Publication year - 2002
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.2002.00586.x
Subject(s) - separase , microbiology and biotechnology , kinetochore , spindle pole body , biology , securin , anaphase , mitosis , cohesin , spindle apparatus , genetics , cell division , cell cycle , chromatin , dna , gene , cell , chromosome
Background: The separase‐securin complex is required for anaphase. Separase activated by securin destruction cleaves the cohesin subunit Scc1/Rad21 enriched in kinetochores. Fission yeast Cut1/separase resides in interphase cytoplasm and mobilizes to the spindle and the spindle pole bodies (SPBs) in mitosis, while Cut2/securin remains in the nucleus from interphase to metaphase, and temporarily locates at the short spindle. Results: We here report a novel SPB‐led dynamic nuclear movement in fission yeast, when the Cut1 C‐terminal fragment is over‐expressed. The tip of the pointed nucleus contained both SPB and centromeric DNA, and rapidly moved along the bundled cytoplasmic microtubules. The same pointed nucleus was produced when the human separase C‐fragment was over‐expressed. The pointed nuclear formation did not require the protease site of separase, but required the conserved C‐terminus and a microtubule‐ and kinetochore‐binding protein Mtc1/Alp14, a homologue of frog XMAP215 and budding yeast Stu2. The movement‐inducing C‐fragment should be cytoplasmic, as the pointed nucleus was abolished when the fragment contained the NLS (nuclear localization signal). Conclusions: Overproduced separase C‐fragment abolishes correct SPB‐positioning in interphase. Resulting pointed nuclear formation (alternatively called ‘pigtail movement’) requires cytoplasmic microtubules and Mtc1/Alp14.