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Spectral imaging fluorescence microscopy
Author(s) -
Haraguchi Tokuko,
Shimi Takeshi,
Koujin Takako,
Hashiguchi Noriyo,
Hiraoka Yasushi
Publication year - 2002
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.2002.00575.x
Subject(s) - fluorescence , förster resonance energy transfer , microscope , fluorescence in the life sciences , microscopy , fluorescence microscope , confocal , 4pi microscope , spectral imaging , optics , resolution (logic) , fluorescence cross correlation spectroscopy , fluorescein isothiocyanate , fluorescence lifetime imaging microscopy , spectral resolution , fluorescein , materials science , laser induced fluorescence , spectral line , physics , multiphoton fluorescence microscope , computer science , astronomy , artificial intelligence
The spectral resolution of fluorescence microscope images in living cells is achieved by using a confocal laser scanning microscope equipped with grating optics. This capability of temporal and spectral resolution is especially useful for detecting spectral changes of a fluorescent dye; for example, those associated with fluorescence resonance energy transfer (FRET). Using the spectral imaging fluorescence microscope system, it is also possible to resolve emitted signals from fluorescent dyes that have spectra largely overlapping with each other, such as fluorescein isothiocyanate (FITC) and green fluorescent protein (GFP).