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A deubiquitinating enzyme, UCH/CeUBP130, has an essential role in the formation of a functional microtubule‐organizing centre (MTOC) during early cleavage in C. elegans
Author(s) -
Lee Jungsoo,
Jee Changhoon,
Lee Jin Il,
Lee Myon Hee,
Lee Moon Hee,
Koo HyeonSook,
Chung Chin Ha,
Ahnn Joohong
Publication year - 2001
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.2001.00471.x
Subject(s) - biology , microbiology and biotechnology , deubiquitinating enzyme , microtubule organizing center , microtubule , caenorhabditis elegans , ubiquitin , genetics , centrosome , gene , cell cycle
Background Deubiquitinating enzymes generate monomeric ubiquitin in protein degradation pathways and are known to be important for the early development in many organisms. Results RNA interference experiments targeted for a UBP homologue, UCH/CeUBP130, in C. elegans resulted in cell division defective embryos. Immunostaining localized UCH/CeUBP130 in the sperm and at the microtubule‐organizing centre (MTOC) during early cleavage. Furthermore, the embryonic lethal phenotype was rescued by mating with wild‐type males. Conclusions Since it is known that the MTOC in the fertilized embryo is contributed by sperm asters in C. elegans , we suggest that UCH/CeUBP130 and ubiquitin protein degradation pathways may be involved in microtubule‐based sperm aster formation. Therefore UCH/CeUBP130 is necessary for the formation of a functional MTOC in the fertilized embryo of C. elegans .