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Mouse Ror2 receptor tyrosine kinase is required for the heart development and limb formation
Author(s) -
Takeuchi Shigeto,
Takeda Kiyoshi,
Oishi Isao,
Nomi Masashi,
Ikeya Makoto,
Itoh Kyoko,
Tamura Shingo,
Ueda Takahiro,
Hatta Toshihisa,
Otani Hiroki,
Terashima Toshio,
Takada Shinji,
Yamamura Hirohei,
Akira Shizuo,
Minami Yasuhiro
Publication year - 2000
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.2000.00300.x
Subject(s) - biology , heart development , ossification , in situ hybridization , anatomy , receptor tyrosine kinase , limb development , limb bud , calcification , dwarfism , nervous system , mutant , endocrinology , receptor , medicine , microbiology and biotechnology , genetics , embryo , neuroscience , embryonic stem cell , gene , messenger rna
Backgrounds A mouse receptor tyrosine kinase (RTK), mRor2, which belongs to the Ror‐family of RTKs consisting of at least two structurally related members, is primarily expressed in the heart and nervous system during mouse development. To elucidate the function of mRor2, we generated mice with a mutated mRor2 locus. Results Mice with a homozygous mutation in mRor2 died just after birth, exhibiting dwarfism, severe cyanosis, and short limbs and tails. Whole‐mount in situ hybridization analysis showed that mRor2 was expressed in the branchial arches, heart and limb/tailbuds, in addition to the developing nervous system. The mutants had cardiac septal defects, mainly a ventricular septal defect. In addition, an examination of the skeletal systems revealed that the mutants had shorter limbs, vertebrae and facial structure, with a particular defect in their distal portions, and that almost no calcification was observed in their distal limbs. Histological examination showed abnormalities in the chondrocytes. Conclusions Our findings suggest that mRor2 plays essential roles in the development of the heart and in limb/tail formation, in particular cardiac septal formation and ossification of distal portions of limbs and tails.