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The G10BP‐1 gene encoding a GC box binding protein, is a target of Myc and Jun/Fos
Author(s) -
Shirasuna Kenna,
Takeuchi Arata,
Bando Takayoshi,
Nakajima Takuma,
Oda Kinichiro
Publication year - 1999
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.1999.00258.x
Subject(s) - ap 1 transcription factor , biology , microbiology and biotechnology , promoter , gene , tata box , complementary dna , oligonucleotide , primer (cosmetics) , transcription (linguistics) , transcription factor , caat box , gene expression , genetics , chemistry , linguistics , philosophy , organic chemistry
Background G10BP, a serum‐inducible factor, represses the transcription of the fibronectin gene through binding to the G‐rich sequences in the promoter excluding Sp1 from binding to these sequences. Results The 5′ flanking sequence of the G10BP‐1 gene was isolated by polymerase chain reaction of the adaptor‐ligated genomic DNA library using the adaptor primer and the G10BP‐1 cDNA primer. The elements required for activation of the G10BP‐1 promoter following serum stimulation were analysed by transfection of quiescent rat 3Y1 cells with G10BP‐1 promoter‐luciferase cDNA constructs containing 5′ sequential deletions or base substitutions. The results showed that the promoter was activated by Myc and Jun through the E box and AP1 sites. The formation of DNA–protein complexes with 32 P‐labelled oligonucleotides containing the E box or AP1 site with cell extracts prepared during G 1 progression was correlated with the promoter activation and greatly reduced by immunodepletion of Myc or c‐Jun from the extracts. Conclusion These results indicate that the G10BP‐1 gene is a target of Myc and Jun/Fos and that these factors repress the fibronectin gene expression through induction of G10BP‐1 during G 1 ‐to‐S phase progression.