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Differential roles of GATA‐1 and GATA‐2 in growth and differentiation of mast cells
Author(s) -
Harigae Hideo,
Takahashi Satoru,
Suwabe Naruyoshi,
Ohtsu Hiroshi,
Gu Lin,
Yang Zhouying,
Tsai FongYing,
Kitamura Yukihiko,
Douglas Engel James,
Yamamoto Masayuki
Publication year - 1998
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.1998.00166.x
Subject(s) - mast cell , biology , progenitor cell , gata2 , interleukin 33 , cellular differentiation , microbiology and biotechnology , phenotype , bone marrow , gene , immunology , haematopoiesis , stem cell , genetics , cytokine , interleukin
Background While mast cells have been previously shown to express both GATA‐1 and GATA‐2 mRNAs, individual functions for these related factors during their course of differentiation within the mast cell lineage have not yet been defined. To address this question, the expression of GATA‐1 and GATA‐2 mRNAs and proteins were examined in three mouse mast cell progenitor lines as well as in mast cells isolated from both wild‐type and GATA‐1‐deficient mice. Results Both mast cell progenitor lines, as well as primary mouse bone marrow‐derived mast cells (BMMCs) and peritoneal mast cells (PMCs) were examined by RNA blotting and immunological analyses. GATA‐2 protein was abundantly expressed in all three mast cell lines and in BMMCs, but only weakly in some of PMCs. In contrast, GATA‐1 protein was expressed in PMCs and BMMCs after culture in the presence of IL‐3 and SCF. We also found the presence of Alcian blue staining‐positive but berberine staining‐negative mast cells in the skin of mice heterozygous to GATA‐1 knock‐down allele. Conclusion These results suggest that the expression of GATA factor‐dependent genes is regulated by GATA‐2 during mast cell development and that GATA‐1 is required for the specification of differentiated mast cell phenotypes.