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Replication profile of Saccharomyces cerevisiae chromosome VI
Author(s) -
Friedman Katherine L.,
Brewer Bonita J.,
Fangman Walton L.
Publication year - 1997
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.1997.1520350.x
Subject(s) - biology , replication timing , genetics , chromosome , origin of replication , genome , dna replication , saccharomyces cerevisiae , control of chromosome duplication , telomere , replication (statistics) , autonomously replicating sequence , pre replication complex , dna , gene , virology
Background: An understanding of the replication programme at the genome level will require the identification and characterization of origins of replication through large, contiguous regions of DNA. As a step toward this goal, origin efficiencies and replication times were determined for 10 ARSs spanning most of the 270 kilobase (kb) chromosome VI of Saccharomyces cerevisiae . Results: Chromosome VI shows a wide variation in the percentage of cell cycles in which different replication origins are utilized. Most of the origins are activated in only a fraction of cells, suggesting that the pattern of origin usage on chromosome VI varies greatly within the cell population. The replication times of fragments containing chromosome VI origins show a temporal pattern that has been recognized on other chromosomes—the telomeres replicate late in S phase, while the central region of the chromosome replicates early. Conclusions: As demonstrated here for chromosome VI, analysis of the direction of replication fork movement along a chromosome and determination of replication time by measuring a period of hemimethylation may provide an efficient means of surveying origin activity over large regions of the genome.