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Phage DNA packaging
Author(s) -
Fujisawa Hisao,
Morita Miyo
Publication year - 1997
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.1997.1450343.x
Subject(s) - concatemer , biology , dna , dna clamp , protein subunit , biochemistry , in vitro recombination , circular bacterial chromosome , microbiology and biotechnology , replisome , dna replication , gene , molecular cloning , peptide sequence , reverse transcriptase , rna , genome
Phage DNA packaging occurs by DNA translocation into a preformed protein shell—a prohead—with the aid of a packaging enzyme or a terminase. The packaging enzyme is composed of two subunits: the large subunit has ATP‐binding, prohead binding, and DNA cleavage activities, and the small subunit is a DNA binding protein. DNA translocation is driven by ATP hydrolysis. In general, phage DNA replication mechanisms lead to the accumulation of concatemers. Concatemers are processed to mature DNA during and depending upon DNA packaging. This review will focus on the molecular mechanism of concatemer processing and the coupling of ATP hydrolysis to DNA translocation.