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A complete Not I restriction map covering the entire long arm of human chromosome 11
Author(s) -
Hosoda Fumie,
Arai Yasuhito,
Kitamura Eiko,
Inazawa Johji,
Fukushima Michiyo,
Tokino Takashi,
Nakamura Yusuke,
Jones Carol,
Kakazu Naoki,
Abe Tatsuo,
Ohki Misao
Publication year - 1997
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.1997.1250324.x
Subject(s) - biology , genetics , restriction map , gene map , gene mapping , positional cloning , chromosome , restriction site , human genome , autosome , restriction enzyme , contig , chromosome 22 , genome , gene , locus (genetics) , nucleic acid sequence
Background: Human chromosome 11 is one of the autosomes on which many disease genes have been mapped. Many different types of map, including a radiation hybrid map, a genetic map, and an STS‐content YAC map, have been constructed for the chromosome. However, a physical map providing accurate physical distances has not yet been established. A chromosome‐wide Not I restriction map was constructed to understand the overall feature of the genome organization and to facilitate the positional cloning of disease genes. Results: A complete Not I restriction map of the entire long arm of human chromosome 11 was constructed using linking‐clone mapping. This physical map covers 77.6 Mb, from a pericentromeric Not I site to the terminus, and provides the most accurate ordering and distance estimation to date. We also mapped 138 sequence markers in the q13 region that have been poorly mapped previously. Conclusions: The restriction map of the entire long arm of human chromosome 11 is the longest restriction map of the human genome. This mapping has disclosed unique features regarding the organization of the chromosome, indicating that restriction sites of Not I, a CpG‐recognition enzyme, are primarily distributed in R (or T) bands and that genetic distance is considerably longer in R (or T) bands than in G bands. The mapping, as well as the dense concentration of mapped markers within the q13 region, should help with positional cloning of the genes associated with various diseases.