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Identification of putative downstream genes of Oct‐3, a pluripotent cell‐specific transcription factor
Author(s) -
Saijoh Yukio,
Fujii Hideta ,
Meno Chikara,
Sato Mayumi,
Hirota Yoshihiro,
Nagamatsu Shinya,
lkeda Masako,
Hamada Hiroshi
Publication year - 1996
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.1996.d01-237.x
Subject(s) - biology , induced pluripotent stem cell , pou domain , gene , embryonic stem cell , transcription factor , genetics , microbiology and biotechnology , gene expression , regulation of gene expression , homeobox
Background : Oct‐3, a pluripotent cell‐specific POU transcription factor, appears to be a key regulator in pluripotential early embryonic cells and germ cells. In order to study how pluripotency is maintained, it is essential to know what genes are regulated by Oct‐3 Results : By employing a subtraction method, we identified several pluripotent cell‐specific genes. Based upon expression patterns in various cell lines lacking or possessing Oct‐3 function, about half of the genes were placed downstream of Oct‐3. These downstream genes included a previously‐known gene (Glut‐3: a gene for a glucose transporter) and novel genes (226, 383 and 880). Their expression patterns paralleled that of Oct‐3: all of these genes were highly expressed in pluripotent cells such as EC/ES cells, but switched off upon differentiation. More importantly, their expression was rescued in 'revertant' cells that ectopically acquired the Oct‐3 transactivating function. Furthermore, the expression profiles of Glut‐3, 226 and 383 during mouse development also overlapped that of Oct‐3. The Glut‐3 gene possessed multiple Oct‐3 binding sites in its transcriptional regulatory regions, suggesting that at least one of the downstream genes was a direct target of Oct‐3. Conclusion : A large proportion of pluripotent cell‐specific genes appear to be downstream targets of Oct‐3.