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Nik1: a Nim1‐like protein kinase of S. cerevisiae interacts with the Cdc28 complex and regulates cell cycle progression
Author(s) -
Tanaka Seiji,
Nojima Hiroshi
Publication year - 1996
Publication title -
genes to cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.912
H-Index - 115
eISSN - 1365-2443
pISSN - 1356-9597
DOI - 10.1046/j.1365-2443.1996.d01-213.x
Subject(s) - cyclin dependent kinase 1 , schizosaccharomyces pombe , biology , cyclin dependent kinase , cell cycle , saccharomyces cerevisiae , cyclin dependent kinase 7 , schizosaccharomyces , microbiology and biotechnology , cell cycle protein , protein kinase a , kinase , yeast , genetics , cell , cyclin dependent kinase 2
Background: The eukaryotic cell cycle is driven by CDK‐cyclin complexes. A number of proteins interact either with CDK or the CDK complex to regulate CDK activity. A search for novel cell cycle regulators in the budding yeast Saccharomyces cerevisiae yielded multicopy suppressors of the cdc2‐L7 mutation of the fission yeast, Schizosaccharomyces pombe . Results: One of the isolated genes was found to encode a putative protein kinase similar to Nim1 of S. pombe and was termed NIK1 (Nim1‐like kinase 1). Transcription of NIK1 ‐was periodic and peaked at the G1/S boundary. Although NIK1 is not essential, Δ nik1 cells showed G2 delay and hydroxyurea (HU) sensitivity. Anomalously elongated buds were observed in the stationary phase or in the presence of HU. Moreover, DNA was aberrantly distributed in the Δ nik1 cdc28 double mutant. Genetical and biochemical evidence suggests that Nik1 interacts with the Cdc28 complex. Conclusions: Nik is a structural and functional homo‐logue of Nim1. Nik1 interacts with the Cdc28 complex and functions not only at the G2/M transition but also at other points of the cell cycle.