Premium
Inhibition of intracellular tumour necrosis factor (TNF)‐α and interleukin (IL)‐6 production in human monocytes by iloprost
Author(s) -
Czeslick E. G.,
Simm A.,
Grond S.,
Silber R.E.,
Sablotzki A.
Publication year - 2003
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1046/j.1365-2362.2003.01241.x
Subject(s) - iloprost , tumor necrosis factor alpha , tumor necrosis factor α , intracellular , interleukin , cytokine , medicine , immunology , necrosis , interleukin 1β , endocrinology , biology , microbiology and biotechnology , prostacyclin
Background To investigate the effects of iloprost as a stable prostacyclin analogue on intracellular expression of IL‐6 and TNF‐α of lipopolysaccharide (LPS)‐stimulated human monocytes in a whole blood system assessed by flow cytometry. Material and methods Whole blood of six healthy volunteers processed immediately after withdrawal and twice on different days (six measurements per experiment) was stimulated in two different settings with LPS (final concentrations 0·2 ng mL −1 and 10 ng mL −1 ) and incubated with iloprost (final concentrations in each experiment were 0·01 n m , 0·1 n m , 0·3 n m , 1 n m , 3 n m , 10 n m , 30 n m and 100 n m ) for 3 h at 37 °C and 5% CO 2 . Intracellular expression of IL‐6 and TNF‐α was assessed by flow cytometry. Results Our investigations showed, for the first time, that iloprost (0·1 n m up to 100 n m ) caused a dose‐dependent inhibitory effect of IL‐6 production in human monocytes stimulated with LPS (10 ng mL −1 ), which was even more obvious in monocytes stimulated with lower concentrated LPS (0·2 ng mL −1 ). Iloprost (0·1 n m up to 100 n m ) was found to inhibit TNF‐α production of LPS‐stimulated monocytes in a dose‐dependent fashion not influenced by LPS concentration. Conclusions Apart from the vasodilatory and antithrombotic effects, iloprost may also down‐regulate the intracellular expression of IL‐6 and TNF‐α in human monocytes.