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Recovery of T‐cell subsets after antiretroviral therapy in HIV‐infected children
Author(s) -
Resino S.,
Bellón J. Ma.,
Gurbindo D.,
León J. A.,
MuñozFernández Ma. Á.
Publication year - 2003
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1046/j.1365-2362.2003.01168.x
Subject(s) - cd38 , cd8 , t cell , flow cytometry , immunology , cd28 , immune system , cytotoxic t cell , immunophenotyping , biology , medicine , cd34 , microbiology and biotechnology , genetics , stem cell , in vitro
Background Variation of percentageCD4+ T cells may accurately reflects the kinetics of a comprehensive immune recovery independently of the antiretroviral (ART) regimen. To test this we have investigated the relationship among peripheral blood T‐cell subsets with the variation of percentageCD4+ T cells during follow up in 49 HIV‐infected children. Methods Children were divided into two groups according to the sign of slope percentageCD4+ T cell during follow up: Ps‐group (positive slope) and Ns‐group (negative percentageCD4 slope) indicative of immunological recovery or not, respectively. CD4+ and CD8+ T‐cell subset percentages were examined by three‐colour flow cytometry. Results We found higher memory CD4+ and CD8+ T‐cell percentages in the Ns‐group than in the Ps‐group, and inversely, higher naive CD4+ and CD8+ T cells in the Ps‐group than in the Ns‐group. CD4+ and CD8+ subsets in the Ps‐group expressed higher levels of CD38+ and lower levels of HLA‐DR+ compared with the Ns‐group. We found a very strong positive correlation among the slope of percentageCD4+, CD4+ CD38+, whereas a negative correlation among the slope of percentageCD4+, the CD8+ CD28+ CD57+ and CD8+ CD57+ T‐cell subsets. Conclusion Recovery of the CD4+ T‐cell percentage induced by ART reflects a reduction in the chronic immune activation and a measurable reconstitution of the immune system and depends on naive CD4+ T cells.