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Lipopolysaccharide represses cholesterol 7‐alpha hydroxylase and induces binding activity to the bile acid response element II
Author(s) -
Dikopoulos N.,
Weidenbach H.,
Adler G.,
Schmid R. M.
Publication year - 2003
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1046/j.1365-2362.2003.01079.x
Subject(s) - cholesterol 7 alpha hydroxylase , farnesoid x receptor , bile acid , nuclear receptor , medicine , response element , endocrinology , chemistry , cholesterol , g protein coupled bile acid receptor , biology , biochemistry , transcription factor , gene expression , promoter , gene
Background Inflammatory states such as hepatitis and sepsis are frequently associated with alterations of bile acid synthesis. These conditions are mediated by bacterial wall products like lipopolysaccharide (LPS). Cholesterol 7‐alpha hydroxylase is the rate‐limiting enzyme of bile acid synthesis. Hydrophobic bile acids repress cholesterol 7‐alpha hydroxylase transcription via binding to the farnesoid X receptor and interaction with the bile acid response element II in the cholesterol 7‐alpha hydroxylase promoter. Methods We tested the effect of LPS on hepatic expression of cholesterol 7‐alpha hydroxylase in C57BL/6 mice and Wistar rats. Further, we analyzed the binding activity of hepatic nuclear extracts to the bile acid response element II and the binding site for farnesoid X receptor heterodimers (ecdysone response element). Result Lipopolysaccharide caused a 100‐fold reduction of cholesterol 7‐alpha hydroxylase mRNA levels in mice and a 10‐fold reduction in rats. Protein levels of cholesterol 7‐alpha hydroxylase also decreased in both species. These changes inversely correlated with the increased binding activity of nuclear proteins to the bile acid response element II and the ecdysone response element. Conclusion Lipopolysaccharide‐induced repression of cholesterol 7‐alpha hydroxylase occurs at the transcriptional level. The underlying mechanism involves an increased binding activity of nuclear proteins to the bile acid response element II and the ecdysone response element. In conclusion, the farnesoid and retinoid X receptors participate in LPS‐induced cholesterol 7‐alpha hydroxylase repression.