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Immunophenotypic analyses of CD34 + cell subsets in bone marrow from HIV‐infected patients during highly‐ active antiretroviral therapy
Author(s) -
Müller F.,
Tjønnfjord G. E.,
Nordøy I.,
Kvale D.,
Mellbye O. J.,
Aukrust P.,
Frøland S. S.
Publication year - 2002
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1046/j.1365-2362.2002.00995.x
Subject(s) - bone marrow , cd34 , myeloid , haematopoiesis , lymphopoiesis , immunology , progenitor cell , medicine , myelopoiesis , stem cell , biology , genetics
Background Because increased bone marrow lymphopoiesis might contribute to immunologic reconstitution during highly‐active antiretroviral therapy (HAART), we examined the effect of HAART on CD34 + cell subsets in bone marrow from HIV‐infected patients. Materials and Methods In 12 HIV‐infected patients, bone marrow and peripheral blood were collected before then 4 and 26 weeks after initiating HAART. Bone marrow in 28 HIV‐seronegative controls was also examined. Immunophenotypic analyses of CD34 + cell subsets in bone marrow were performed by flow cytometry. Results Our main findings in bone marrow were: (i) HIV‐infected patients had increased proportions of CD34 + cells expressing T‐ and B‐cell markers before initiating HAART; (ii) in contrast, these patients had decreased proportions of CD34 + cells expressing myeloid‐associated markers; (iii) although HAART induced an increase in peripheral T‐cell counts, the percentage of CD34 + cells expressing T‐cell markers tended to decrease during such therapy; (iv) HAART induced a decrease in serum IgG accompanied by a slight decrease in the proportion of CD34 + cells expressing B‐cell markers; (v) in contrast, HAART induced a significant increase in peripheral granulocyte counts, accompanied by a slightly increased proportion of CD34 + cells expressing myeloid‐associated molecules. Conclusion Our findings are compatible with an HIV‐related block in T‐cell differentiation, leading to accumulation of T‐cell progenitors in bone marrow, and such a block may be removed by HAART.