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Food deprivation increases post‐heparin lipoprotein lipase activity in humans
Author(s) -
Ruge T.,
Svensson A.,
Eriksson J. W.,
Olivecrona T.,
Olivecrona G.
Publication year - 2001
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1046/j.1365-2362.2001.00930.x
Subject(s) - lipoprotein lipase , heparin , endocrinology , medicine , adipose tissue , guinea pig , chemistry , lipase , hepatic lipase , insulin , enzyme , biochemistry
Objective To study the effect of fasting on lipoprotein lipase (LPL) activity in human post‐heparin plasma, representing the functional pool of LPL. Design Fourteen healthy volunteers were recruited for the study. The subjects were fasted for 30 h. Activities of LPL and hepatic lipase (HL), and LPL mass, were measured in pre‐ and post‐heparin plasma in the fed and in the fasted states, respectively. For comparison, LPL and HL activities were measured in pre‐ and post‐heparin plasma from fed and 24‐h‐fasted guinea pigs. Results Fasting caused a significant drop in the levels of serum insulin, triglycerides and glucose in the human subjects. Post‐heparin LPL activity increased from 79 ± 6·4 mU mL −1 in the fed state to 112 ± 10 mU mL −1 in the fasted state ( P < 0·01), while LPL mass was 361 ± 29 in the fed state and 383 ± 28 in the fasted state, respectively ( P = 0·6). In contrast, fasting of guinea pigs caused an 80% drop in post‐heparin LPL activity. The effect of fasting on human and guinea pig post‐heparin HL activity were moderate and statistically not significant. Conclusions In animal models such as rats and guinea pigs, post‐heparin LPL activity decreases on fasting, presumably due to down‐regulation of adipose tissue LPL. In humans, fasting caused increased post‐heparin LPL activity.