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Subcutaneous adipose tissue expression of tumour necrosis factor‐ α is not associated with whole body insulin resistance in obese nondiabetic or in type‐2 diabetic subjects
Author(s) -
Heikki A. Koistinen,
J.-P. Bastard,
Eric Dusserre,
Pertti Ebeling,
N Zegari,
Fabrizio Andréelli,
Claude Jardel,
M. Donner,
Laurent Meyer,
Philippe Moulin,
Bernard Hainque,
J. P. Riou,
Martine Laville,
Veikko A. Koivisto,
Hubert Vidal
Publication year - 2000
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1046/j.1365-2362.2000.00625.x
Subject(s) - adipose tissue , medicine , endocrinology , insulin resistance , obesity , type 2 diabetes , diabetes mellitus , tumor necrosis factor alpha , glucose clamp technique , insulin , pancreatic hormone
Background An association with subcutaneous adipose tissue TNF α expression and insulin resistance has been suggested in obesity/type‐2 diabetes, but this has not been examined directly. In the first part of the study we investigated whether this association is present in 7 lean, 10 obese nondiabetic and 9 type‐2 diabetic men. In the second part of the study we examined the relationship between adipose tissue TNF α mRNA levels and BMI in 81 nondiabetic subjects spanning a wide range of BMIs. Methods Subcutaneous adipose tissue TNF α mRNA levels and insulin sensitivity were determined with quantitative RT‐competitive PCR and hyperinsulinaemic clamp, respectively. Results. Subcutaneous adipose tissue TNF α mRNA levels were similar in 7 lean and 10 obese nondiabetic and 9 type‐2 diabetic men ( P = 0.68), and did not change in response to 240‐min hyperinsulinaemia. TNF α mRNA levels and insulin sensitivity were not correlated. Unexpectedly, no correlation between TNF α mRNA and BMI was found. The relationship between adipose tissue TNF α mRNA and BMI was examined further in 31 male and 50 female nondiabetic subjects. The subcutaneous adipose tissue TNF α mRNA level correlated with BMI in all subjects ( r S = 0.32, P < 0.01), and in a subgroup analysis in men ( r S = 0.55, P < 0.01) but not in women ( r S = − 0.08). The correlation in men was dependent on a fourfold higher TNF α mRNA level in 5 morbidly obese men while there was no difference in TNF α mRNA levels in lean or obese men. Conclusions Subcutaneous adipose tissue TNF α expression does not correlate with insulin sensitivity in nondiabetic or type‐2 diabetic men; is not regulated by acute hyperinsulinaemia; and is increased only in morbidly obese men.