Premium
Assessment of human muscle glycogen synthesis and total glucose content by in vivo 13 C MRS
Author(s) -
A.J. van den Bergh,
Cees J. Tack,
H.J. van den Boogert,
G.M.M. Vervoort,
Paul Smits,
Arend Heerschap
Publication year - 2000
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1046/j.1365-2362.2000.00603.x
Subject(s) - glycogenesis , glycogen , medicine , endocrinology , in vivo , insulin , glucose clamp technique , glycogen synthase , skeletal muscle , glucose uptake , chemistry , carbohydrate , biology , pancreatic hormone , insulin resistance , microbiology and biotechnology
Background Obesity is often accompanied by a decreased ability of insulin to stimulate glucose uptake and glycogenesis in skeletal muscle. The aim of this study was to investigate the rate of glycogen formation and of muscular glucose content in relation to insulin sensitivity under euglycemic conditions. Materials and methods We applied a hyperinsulinemic (430 pmol m −2 min −1 ) euglycemic clamp with infusion of 20% glucose (30% enriched with 13 C‐1‐glucose) to 8 subjects with a wide range of insulin sensitivities. Glycogen and glucose levels were monitored simultaneously by in vivo 13 C MRS of the calf muscle on a clinical MR system at 1.5T field strength. Results and conclusions Glycogen synthesis rate showed a strong correlation with whole body glucose uptake during the clamp ( r = 0.93, P < 0.01). With the use of 13 C MRS, total muscular glucose content could be determined in vivo , and showed a positive, linear correlation with glycogen synthesis rate ( r = 0.85, P < 0.01). 13 C MRS provides important information regarding in vivo insulin action. Preliminary results indicate that the glycogen synthesis rate improves after treatment with troglitazone.