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Energetics and function of the failing human heart with dilated or hypertrophic cardiomyopathy
Author(s) -
Kalsi K. K.,
Smolenski R. T.,
Pritchard R. D.,
Khaghani A.,
Seymour AM. L.,
Yacoub M. H.
Publication year - 1999
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1046/j.1365-2362.1999.00468.x
Subject(s) - phosphocreatine , medicine , contractility , hypertrophic cardiomyopathy , dilated cardiomyopathy , heart failure , creatine , heart transplantation , adenine nucleotide , high energy phosphate , cardiomyopathy , cardiology , glycolysis , endocrinology , creatine kinase , chemistry , energy metabolism , metabolism , nucleotide , biochemistry , gene
Background Impaired energy metabolism in the failing human heart could be an important mechanism of functional deterioration. The purpose of this study was to assess the changes of myocardial energy metabolism in the human heart at end‐stage heart failure. Materials and methods The left ventricular myocardium of patients undergoing heart transplantation due to dilated (DCM, n = 14) or hypertrophic cardiomyopathy (HCM, n = 5) and non‐diseased donor heart samples ( n = 4) were analysed for citrate synthase (CS), enzymes of the glycolytic pathway as well as concentrations of phosphocreatine (PCr), creatine (Cr), adenine and guanine nucleotides. Results Total creatine levels (phosphocreatine + creatine) were significantly decreased ( P < 0.05) in both groups of diseased hearts (3.87 ± 0.57 in DCM, 5.09 ± 1.23 in HCM compared with control 10.7 ± 3.5 μmol g −1 wet weight). There was a trend for higher guanine nucleotide content in failing hearts, but no significant differences were observed in total adenine nucleotides and total NAD content. CS was markedly reduced ( P < 0.05) in both groups of diseased hearts: in the DCM to 13.8 ± 1.3 μmol min −1 g −1 wet weight, and in HCM to 11.9 ± 2.4 compared with the control 29.2 ± 2.2. Glycolytic enzymes were decreased compared with the control, and this decrease was greater in DCM than in HCM. Echocardiographic indices of contractility were considerably better in hypertrophic cardiomyopathy. Conclusion Despite the different mechanisms of cardiac failure and the differences in contractility of the heart we have observed, metabolic changes are very similar in hypertrophic and dilated cardiomyopathy. Depletion of the creatine pool suggests an alteration in the intracellular energy reserves and transfer, whereas the decrease in citrate synthase activity suggests reduced oxidative capacity in both dilated and hypertrophic cardiomyopathy.