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Lack of interleukin 10 regulation of antigen presentation‐associated molecules expressed on colonic epithelial cells
Author(s) -
Bourreille,
Segain,
Raingeard de la Blétière,
Siavoshian,
Vallette,
Galmiche,
Blottière
Publication year - 1999
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1046/j.1365-2362.1999.00410.x
Subject(s) - antigen presentation , biology , interleukin 22 , antigen , immune system , interleukin , microbiology and biotechnology , cytokine , cd80 , receptor , immunology , cd40 , t cell , cytotoxic t cell , biochemistry , in vitro
Background Colonic epithelial cells may behave as antigen‐presenting cells. Interleukin 10 (IL‐10) is known to play a major role in the intestinal immune system; however, it remains to be determined whether human intestinal epithelial cells express IL‐10 receptor, and whether this cytokine modulates their expression of antigen presentation‐associated molecules Methods The binding of biotinylated IL‐10 was studied in SW 1116, HT‐29 and T84 human colonic epithelial cell lines and freshly isolated normal colonic epithelial cells. Reverse transcription‐polymerase chain reaction was also performed to detect IL‐10 receptor mRNA. The effect of IL‐10 on antigen presentation associated molecules was assessed by flow cytometry. Results Biotinylated IL‐10 bound to SW 1116, HT‐29, T84, and normal colonic epithelial cells. IL‐10 receptor mRNA was detected in SW 1116 and normal epithelial cells. SW 1116 and HT‐29 cells expressed MHC class I and ICAM‐1, but not CD80, and SW 1116 constitutively expressed HLA‐DR. Interferon‐γ up‐regulated HLA‐DR and ICAM‐1 expression on both cells, whereas lipopolysaccharide increased ICAM‐1 expression only on SW 1116. IL‐10 failed to modulate these antigens, even after stimulation by lipopolysaccharide or interferon‐γ. Moreover, these molecules decreased IL‐10 binding in both lines. Conclusion The presence of IL‐10 receptor on intestinal epithelial cells suggest that IL‐10 may play a role in mucosal physiology, however its effect on the immune response remains to be determined.