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Varying very low‐density lipoprotein secretion of rat hepatocytes by altering cellular levels of calcium and the activity of protein kinase C
Author(s) -
O G Björnsson,
Catherine S. Bourgeois,
Geoffrey F. Gibbons
Publication year - 1998
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1046/j.1365-2362.1998.00354.x
Subject(s) - secretion , calcium , chemistry , microbiology and biotechnology , protein kinase a , endocrinology , protein kinase c , kinase , medicine , hepatocyte , biology , biochemistry , in vitro
Background Calcium antagonists lower plasma levels of lipoproteins and suppress hepatic very low‐density lipoprotein (VLDL) secretion. Similar effects have been observed with the calcium ionophore A23187. We studied further the effect of calcium on VLDL metabolism. Methods Hepatocytes from male Wistar rats were isolated and cultured in the presence or absence of calcium‐mobilizing hormones, or compounds that either stimulate or inhibit the activity of protein kinase C. Secreted VLDL ( d < 1.006 g mL −1 ) was isolated by centrifugation (145 000 × g ), and lipids and apolipoprotein B were analysed. Results VLDL secretion reached maximum in hepatocytes cultured in medium containing calcium 0.8–2.4 mmol L −1 . Depleting the cells of calcium by incubating in calcium‐free medium or by treating the cells with the Ca 2+ ‐ATPase inhibitor thapsigargin (5 × 10 −7 mol L −1 ) suppressed lipid secretion to less than 15% of control, and this was accompanied by an increase in cellular levels of triacylglycerol. Calcium loading (medium calcium > 2.4 mmol L −1 ) suppressed both lipoprotein secretion and cellular levels of lipids, suggesting a reduced overall rate of lipid synthesis. At an extracellular calcium concentration of 0.8 mmol L −1 , angiotensin II, vasopressin, endothelin‐1 (10 −7 mol L −1 ) or phenylephrine (10 −4 mol L −1 ) suppressed VLDL secretion (maximum to 37% of control), and elevated medium calcium attenuated this effect. The protein kinase C inhibitor chelerythrine (5 × 10 −5 mol L −1 ) and the protein kinase C activator phorbol 12‐myristate 13‐acetate (PMA) (10 −6 mol L −1 ), suppressed VLDL secretion to 18% and 60% of control, respectively, whereas the protein kinase C‐inactive 4α‐PMA was without an effect. No effect on ketogenesis was observed by these compounds, indicating that suppressed lipid secretion was not due to an enhanced oxidation of lipids. Conclusions Hepatic VLDL secretion can be related to changes in hepatocyte levels of calcium and the activity of protein kinase C.