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Circulating anti‐(xanthine oxidoreductase) antibodies in healthy human adults
Author(s) -
BENBOUBETRA M.,
GLEESON A.,
HARRIS C. P. D.,
KHAN J.,
ARRAR L.,
BRENNAND D.,
REID J.,
RECKLESS J. D.,
HARRISON R.
Publication year - 1997
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1046/j.1365-2362.1997.1550704.x
Subject(s) - immunogen , antibody , enzyme , biology , immunology , biochemistry , monoclonal antibody
Levels of free anti‐(xanthine oxidoreductase) (XOR) antibodies in the serum of normal healthy human subjects were determined, using both human and bovine enzyme as antigen, in an enzyme‐linked immunosorbent assay (ELISA). Levels of IgM class anti‐(human XOR) antibodies were found to be particularly high (mean values representing approximately 3% of total IgM) and to be significantly higher than levels of IgM anti‐(bovine XOR) antibodies, indicating that endogenous XOR, rather than ingested bovine milk XOR, is the immunogen. IgM anti‐XOR antibody levels were significantly higher in women under 50 years than in age‐matched men, or in older women. Levels of IgG class anti‐XOR antibodies were much lower and showed no correlation with gender or age. Affinity‐purified anti‐(human XOR) antibodies only partially inhibited enzymic activities of XOR. The majority of both IgM and IgG anti‐(human XOR) antibodies in serum occurred as immune complexes, suggesting that the specific antibodies have a protective role in removing potentially damaging XOR from the circulation.