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Butyrate enhances major histocompatibility complex class I, HLA‐DR and ICAM‐1 antigen expression on differentiated human intestinal epithelial cells
Author(s) -
SIAVOSHIAN S.,
BLOTTIÈRE H. M.,
BENTOUIMOU N.,
CHERBUT C.,
GALMICHE J. P.
Publication year - 1996
Publication title -
european journal of clinical investigation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.164
H-Index - 107
eISSN - 1365-2362
pISSN - 0014-2972
DOI - 10.1046/j.1365-2362.1996.2180561.x
Subject(s) - butyrate , antigen , human leukocyte antigen , antigen processing , antigen presentation , biology , major histocompatibility complex , histocompatibility , cell culture , immunology , microbiology and biotechnology , mhc class i , t cell , immune system , biochemistry , genetics , fermentation
Besides its metabolic role, butyrate, a by‐product of colonic fermentation, can modulate colonocyte proliferation and the expression of various molecules. In inflammatory bowel diseases, epithelial cells express HLA class II molecules and may behave as antigen‐presenting cells. This study was performed to characterize the effect of butyrate on major histocompatibility complex expression by human colonocytes in comparison with interferon‐γ. Five cell lines displaying different differentiation features were analysed for antigen expression by flow cytofluorimetry. All lines expressed class I antigens, whereas only SW 1116 cells express HLA‐DR. On these cells, butyrate and interferon‐γ strongly enhanced HLA‐DR and ICAM‐1 expression, whereas a mild increase in class I antigens was noted. Moreover, an increase in class I antigens was observed on two other differentiated cell lines, and it was synergistic with interferon‐γ. Butyrate, by its modulation of HLA‐DR, ICAM‐1 and HLA class I expression, may act on antigen presentation and, thus, influence some inflammatory processes.